Whole-Cell Patch-Clamp and Fluorescence Imaging of SuM Neurons in Mouse Brain Slices
ID: 001510
DRAFT
DRAFT
Contact Krook-Magnuson, Esther
File Count 215
Size 24.2 GB
Created June 17, 2025
Last update September 3, 2026
Licenses: spdx:CC-BY-4.0
Access Information: dandi:OpenAccess
This dandiset contains whole-cell patch-clamp recordings and confocal fluorescence images from the supramammillary nucleus (SuM) in acute mouse brain slices. Whole-cell recordings were made from fluorescently identified SuM neurons (including SuM cells projecting to the hippocampus) and comprise both current-clamp firing-property measurements and voltage-clamp light-evoked synaptic responses...
Keywords:
supramammillary nucleus
SuM
hippocampus
whole-cell patch-clamp
slice electrophysiology
optogenetics
GABAergic transmission
synaptic terminals
puncta quantification
confocal microscopy
SypEGFP
mouse
Funding information
No funding information provided.
Anatomy
No anatomical information provided.
This Dandiset does not specify which brain regions or anatomical structures it covers. Adding indexed anatomy (e.g. UBERON terms) in the metadata editor makes this dataset discoverable by anatomical location.
Assets Summary
Approach
microscopy approach; cell population imaging
electrophysiological approach
Data StandardRRID:SCR_016124RRID:SCR_015242
Brain Imaging Data Structure (BIDS)
Neurodata Without Borders (NWB)
Number Of Subjects
62
Variable Measured
OnePhotonSeries
OpticalChannel
ProcessingModule
ImagingPlane
VoltageClampSeries
VoltageClampStimulusSeries
CurrentClampSeries
CurrentClampStimulusSeries
Measurement Technique
one-photon microscopy technique
analytical technique
surgical technique
voltage clamp technique
current clamp technique