Whole-Cell Patch-Clamp and Fluorescence Imaging of SuM Neurons in Mouse Brain Slices
ID: 001510
DRAFT
DRAFT
Contact Krook-Magnuson, Esther
File Count 53
Size 6.2 GB
Created June 17, 2025
Last update July 30, 2026
Licenses: spdx:CC-BY-4.0
Access Information: dandi:OpenAccess
This dandiset contains whole-cell patch-clamp recordings and confocal fluorescence images from the supramammillary nucleus (SuM) in acute mouse brain slices. Whole-cell recordings were made from fluorescently identified SuM neurons (including SuM cells projecting to the hippocampus) and comprise both current-clamp firing-property measurements and voltage-clamp light-evoked synaptic responses...
Keywords:
supramammillary nucleus
SuM
hippocampus
whole-cell patch-clamp
slice electrophysiology
optogenetics
GABAergic transmission
synaptic terminals
puncta quantification
confocal microscopy
SypEGFP
mouse
Funding information
No funding information available.
Assets Summary
Approach
electrophysiological approach
microscopy approach; cell population imaging
Data StandardRRID:SCR_016124RRID:SCR_015242
Brain Imaging Data Structure (BIDS)
Neurodata Without Borders (NWB)
Number Of Subjects
11
Variable Measured
ProcessingModule
VoltageClampSeries
CurrentClampSeries
OnePhotonSeries
OpticalChannel
ImagingPlane
Measurement Technique
voltage clamp technique
current clamp technique
analytical technique
surgical technique
one-photon microscopy technique