Imaging the voltage of neurons distributed across entire brains of larval zebrafish

In this project, we present a remote scanning light-sheet microscope capable of imaging GEVI-expressing neurons distributed throughout entire brains of larval zebrafish at a volumetric rate of 200.8 Hz. We measured voltage of ∼1/3 of the neurons of the brain, distributed throughout. Here the raw light-sheet voltage imaging datasets of larval zebrafish brains, captured using this new microscope,...