Mapping the Neural Dynamics of Locomotion across the Drosophila Brain

Walking is a fundamental mode of locomotion, yet its neural correlates are unknown at brain-wide scale in any animal. We use volumetric two-photon imaging to map neural activity associated with walking across the entire brain of Drosophila. We detect locomotor signals in approximately 40% of the brain, identify a global signal associated with the transition from rest to walking, and define...