Probing subthreshold dynamics of hippocampal neurons by pulsed optogenetics
DOI:
10.48324/dandi.000568/0.230705.1633
10.48324/dandi.000568/0.230705.1633
ID: 000568
0.230705.1633
0.230705.1633
Contact Valero, Manuel
File Count 138
Size 842.6 GiB
Created July 5, 2023
Last update July 5, 2023
Licenses: spdx:CC-BY-4.0
Access Information: dandi:OpenAccess
Understanding how excitatory (E) and inhibitory (I) inputs are integrated by neurons requires monitoring their subthreshold behavior. We probed the subthreshold dynamics using optogenetic depolarizing pulses in hippocampal neuronal assemblies in freely moving mice. Excitability decreased during sharp- wave ripples coupled with increased I. In contrast to this “negative gain,” optogenetic probing...
Subject matter:
hippocampus
Funding information
International Human Frontier Science Program Organization
- Award Number: LT0000717/2018, NIH MH107396, NS 090583 NSF PIRE, 1545858, U19 NS107616, U19 NS104590, LT0000717/2018
National Institutes of Health
- Award Number: MH107396, U19NS107616
National Institute of Mental Health
- Award Number: NS107616
European Molecular Biology Organization
- Award Number: EMBO ALTF 1161-2017
National Science Foundation
- Award Number: NeuroNex MINT grant 1707316
Leon Levy Foundation
- Award Number: NIH MH107396, NS 090583 NSF PIRE, 1545858, U19 NS107616
Related resources
Assets Summary
Approach
behavioral approach
electrophysiological approach
optogenetic approach
Data StandardRRID:SCR_015242
Neurodata Without Borders (NWB)
Number Of Subjects
4
Variable Measured
LFP
Units
SpatialSeries
ElectrodeGroup
ElectricalSeries
Position
ProcessingModule
OptogeneticSeries
Measurement Technique
spike sorting technique
behavioral technique
signal filtering technique
analytical technique
surgical technique
multi electrode extracellular electrophysiology recording technique