Effect of the pulse duration on electroporation by unipolar and 50% bipolar pulses

Trains of 5 different duration pulses, all exposures were at 1 Hz. Cells were electroporated by a train of 5 unipolar 300ns, 1200ns, 50us, 100us, 500us or bipolar 300+300ns, 1200+1200ns, 50+50us, 100+100us, 500+500us pulses (second pulse's amplitude was 50% in relation to the first pulse's amplitude). The electrodes were two parallel stainless-steel cylinders, 1.5 mm in diameter and 3 mm apart....