Simultaneous loose seal cell-attached recordings and two-photon imaging of GCaMP8 expressing mouse V1 neurons with drifting gratings visual stimuli - RAW movies
ID: 000362
DRAFT
DRAFT
Contact Rózsa, Márton
File Count 52
Size 370.2 GiB
Created November 16, 2022
Last update June 20, 2023
Licenses: spdx:CC-BY-4.0
Access Information: dandi:OpenAccess
We tested the jGCaMP8 sensors in L2/3 pyramidal neurons of mouse primary visual cortex. We made a craniotomy over V1 and infected neurons with adeno-associated virus (AAV2/1-hSynapsin-1) encoding jGCaMP8 variants (s/m/f), jGCaMP7f, or XCaMP-Gf. 18-80 days after the virus injection, the mouse was anesthetized, and we surgically removed the cranial window and performed durotomy. The craniotomy was...
Funding information
No funding information available.
Assets Summary
Approach
microscopy approach; cell population imaging
electrophysiological approach
Data StandardRRID:SCR_015242
Neurodata Without Borders (NWB)
Number Of Subjects
11
Variable Measured
ProcessingModule
OpticalChannel
ImagingPlane
TwoPhotonSeries
PlaneSegmentation
CurrentClampStimulusSeries
CurrentClampSeries
VoltageClampSeries
VoltageClampStimulusSeries
Measurement Technique
surgical technique
current clamp technique
two-photon microscopy technique
analytical technique
voltage clamp technique