Simultaneous loose seal cell-attached recordings and two-photon imaging of GCaMP8 expressing mouse V1 neurons with drifting gratings visual stimuli
ID: 000168
DRAFT
DRAFT
Contact Rozsa, Marton
File Count 170
Size 1.4 TB
Created November 11, 2021
Last update June 20, 2023
Licenses: spdx:CC-BY-4.0
Access Information: dandi:OpenAccess
We tested the jGCaMP8 sensors in L2/3 pyramidal neurons of mouse primary visual cortex. We made a craniotomy over V1 and infected neurons with adeno-associated virus (AAV2/1-hSynapsin-1) encoding jGCaMP8 variants (s/m/f), jGCaMP7f, or XCaMP-Gf. 18-80 days after the virus injection, the mouse was anesthetized, and we surgically removed the cranial window and performed durotomy. The craniotomy was...
Keywords:
2-photon
visual cortex
calcium
spike
action potential
layer 2
AAV
adeno-associated virus
jGCaMP8s
jGCaMP8m
jGCaMP8f
jGCaMP7f
XCaMP-Gf
Contributors
Funding information
HHMI
Anatomy
No anatomical information provided.
This Dandiset does not specify which brain regions or anatomical structures it covers. Adding indexed anatomy (e.g. UBERON terms) in the metadata editor makes this dataset discoverable by anatomical location.
Related resources
Assets Summary
Approach
microscopy approach; cell population imaging
electrophysiological approach
Data StandardRRID:SCR_015242
Neurodata Without Borders (NWB)
Number Of Subjects
30
Variable Measured
PlaneSegmentation
CurrentClampStimulusSeries
CurrentClampSeries
TwoPhotonSeries
ProcessingModule
VoltageClampStimulusSeries
VoltageClampSeries
Measurement Technique
two-photon microscopy technique
current clamp technique
analytical technique
voltage clamp technique